1KGF
STRUCTURE OF BETA-LACTAMASE ASN 170 GLN MUTANT
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-12-14 |
| Detector | SIEMENS |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 54.300, 94.800, 140.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.000 |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.200 * | 2.200 * |
| Rmerge | 0.079 | 0.215 * |
| Total number of observations | 28166 * | |
| Number of reflections | 16464 | |
| Completeness [%] | 87.0 | 76 * |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 5-6.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 42 (%sat) | |
| 3 | 1 | drop | PEG2000 | 0.2 (%(w/v)) | |
| 4 | 1 | drop | 0.15 (M) | ||
| 5 | 1 | drop | 0.05 (M) | ||
| 6 | 1 | reservoir | ammonium sulfate | 84 (%sat) | |
| 7 | 1 | reservoir | PEG2000 | 0.4 (%(w/v)) | |
| 8 | 1 | reservoir | 0.3 (M) | ||
| 9 | 1 | reservoir | 0.1 (M) |
