1KGE

STRUCTURE OF BETA-LACTAMASE ASN 170 MET MUTANT

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1995-04-11
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	54.300, 94.000, 139.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.156
Rwork	0.156
RMSD bond length	0.019
RMSD bond angle	1.900
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.090	0.347 *
Total number of observations	89328 *
Number of reflections	19020
Completeness [%]	79.0	42 *
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	5-6.5 (mg/ml)
2	1	drop	ammonium sulfate	42 (%sat)
3	1	drop	PEG1000	0.1 (%(w/v))
4	1	drop		0.15 (M)
5	1	drop		0.05 (M)
6	1	reservoir	ammonium sulfate	84 (%sat)
7	1	reservoir	PEG1000	0.2 (%(w/v))
8	1	reservoir		0.3 (M)
9	1	reservoir		0.1 (M)