1KGD

Crystal Structure of the Guanylate Kinase-like Domain of Human CASK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-22
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	70.341, 70.341, 74.861
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 1.314
R-factor	0.186 *
Rwork	0.186
R-free	0.21244
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	2.371 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.400
High resolution limit [Å]	1.310	1.310
Rmerge	0.034 *	0.145 *
Total number of observations	327011 *
Number of reflections	48853
<I/σ(I)>	20.9	8.31
Completeness [%]	94.2	81
Redundancy	6.7	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	sodium formate, potassium chloride, magnesium chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	4 (M)
2	1	drop	protein	15 (mg/ml)
3	1	drop	Tris-HCl	50 (mM)	pH7.5
4	1	drop		200 (mM)
5	1	drop		5 (mM)
6	1	drop	EDTA	1 (mM)
7	1	drop	dithiothreitol	10 (mM)