1KEX
Crystal Structure of the b1 Domain of Human Neuropilin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-15 |
| Detector | RIGAKU |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.827, 62.827, 88.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.900 |
| Rwork | 0.189 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1czt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.984 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 * | 0.105 * |
| Total number of observations | 370706 * | |
| Number of reflections | 12290 | |
| <I/σ(I)> | 32.1 | |
| Completeness [%] | 97.3 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 4 * | PEG 8000, MES, zinc acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | drop | Tris | 20 (mM) | pH7.9 |
| 5 | 1 | drop | 50 (mM) | ||
| 6 | 1 | drop | TCEP | 0.25 (mM) | |
| 7 | 1 | drop | protein | 10 (mg/ml) |
