1KEN
INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH AN ANTIBODY THAT PREVENTS THE HEMAGGLUTININ LOW PH FUSOGENIC TRANSITION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 143.041, 315.588, 97.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.500 |
| R-factor | 0.256 * |
| Rwork | 0.255 |
| R-free | 0.32400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 25.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.128 * | 0.423 * |
| Number of reflections | 54923 | |
| <I/σ(I)> | 5.3 | 1.8 |
| Completeness [%] | 98.2 | 98.2 |
| Redundancy | 3.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 * | Gigant, B., (2000) Acta Crystallogr., Sect.D, 56, 1067. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.010-0.015 (mg/ml) | |
| 2 | 1 | drop | 0.15 (M) | ||
| 3 | 1 | drop | 0.04 (%(w/v)) | ||
| 4 | 1 | reservoir | PEG20000 | 13-15 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
| 6 | 1 | reservoir | 20 (mM) |
