1KEM
CATALYTIC ANTIBODY 28B4 FAB FRAGMENT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.200, 58.600, 43.400 |
| Unit cell angles | 95.30, 103.20, 93.60 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.875 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.200 | 2.000 |
| Rmerge | 0.078 | 0.210 |
| Total number of observations | 51820 * | |
| Number of reflections | 21852 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 98.0 | 43 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab | 12.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | methionine | 1 (mM) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 20 (%) | |
| 7 | 1 | reservoir | isopropanol | 10 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 0.1 (M) |
