1KDM
THE CRYSTAL STRUCTURE OF THE HUMAN SEX HORMONE-BINDING GLOBULIN (TETRAGONAL CRYSTAL FORM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 110 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.93 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 52.240, 52.240, 148.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.350 |
R-factor | 0.193 * |
Rwork | 0.193 |
R-free | 0.26400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 0.039 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.400 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.091 | 0.379 |
Total number of observations | 72753 * | |
Number of reflections | 8915 | |
Completeness [%] | 94.9 | 99.1 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Grishkovskaya, I., (1999) Acta Crystallog. D55, 2053. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | imidazole-HCl | 80 (mM) | pH7.5 |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | DHT | 0.010 (mM) | |
5 | 1 | reservoir | 2-propanol | 10 (%) | |
6 | 1 | reservoir | PEG4000 | 20 (%) | |
7 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |