1KDK
THE STRUCTURE OF THE N-TERMINAL LG DOMAIN OF SHBG IN CRYSTALS SOAKED WITH EDTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 103.720, 103.720, 85.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.194 * |
| Rwork | 0.194 |
| R-free | 0.24500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d2s |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.037 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 * | 0.370 |
| Total number of observations | 90466 * | |
| Number of reflections | 19262 | |
| Completeness [%] | 98.1 | 99.2 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Grishkovskaya, I., (1999) Acta Crystallog. D55, 2053. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 2.5 (mM) | ||
| 4 | 1 | drop | DHT | 0.003 (mM) | |
| 5 | 1 | reservoir | 2-propanol | 20 (%) | |
| 6 | 1 | reservoir | PEG400 | 10 (%) | |
| 7 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
