1KDH
Binary Complex of Murine Terminal Deoxynucleotidyl Transferase with a Primer Single Stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.960 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.800, 85.000, 111.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jms |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.075 | 0.608 |
| Number of reflections | 8579 | |
| <I/σ(I)> | 8.5 | 2.5 |
| Completeness [%] | 91.5 | 87.4 |
| Redundancy | 2.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | PEG 6K, 1M LiCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6K | ||
| 2 | 1 | 1 | LiCl |
