1KDA

STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	48.200, 48.200, 63.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.900
R-factor	0.189
Rwork	0.189
RMSD bond length	0.006
RMSD bond angle	1.540 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.056 *
Number of reflections	8957 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.15 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	potassium phosphate	10.5 (mM)
2	1	1	protein	2 (mg/ml)
3	1	2	MPD