1KCV

Crystal structure of antibody pc282

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	300
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	52.646, 72.939, 58.550
Unit cell angles	90.00, 100.51, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.203 *
Rwork	0.203
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.379 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Rmerge	0.072	0.471 *
Total number of observations	378203 *
Number of reflections	36471
<I/σ(I)>	10.5
Completeness [%]	91.0	83.2 *
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	300	10 % PEG 3K, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-Cl	50 (mM)	pH7.2
2	1	reservoir	sodium azide	0.05 (%)
3	1	drop	peptide/Fab	10 (mg/ml)