1KC6
HincII Bound to Cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 67.120, 177.670, 256.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.28500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.560 * | 2.600 |
| Rmerge | 0.047 | 0.191 |
| Total number of observations | 91893 * | |
| Number of reflections | 42701 | |
| <I/σ(I)> | 16.6 | 4.2 |
| Completeness [%] | 87.3 | 76 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 290 * | Horton, N.C., (1999) Acta Crystallogr., D55, 1943. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium citrate | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | PEG 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HincII-DNA complex | 7.2 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.5 |
| 4 | 1 | reservoir | 0.12 (M) |
