1KBY

Structure of Photosynthetic Reaction Center with bacteriochlorophyll-bacteriopheophytin heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200HB
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-02-20
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	142.310, 142.310, 187.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.070 - 2.500
R-factor	0.195
Rwork	0.195
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.500	2.550
Rmerge	0.120	0.650
Number of reflections	59548
<I/σ(I)>		1.4
Completeness [%]	83.7	61.7
Redundancy		1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	LDAO, Potassium phosphate, heptano-1,2,3-triol, dioxane, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 25K
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	LDAO, Potassium phosphate, heptano-1,2,3-triol, dioxane, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 25K