1KAW

STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-11-11
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	58.080, 105.850, 90.240
Unit cell angles	90.00, 99.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.23 *
Rwork	0.221
R-free	0.29500 *
RMSD bond length	0.017 *
RMSD bond angle	20.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.85)
Refinement software	X-PLOR (3.85)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.053
Number of reflections	12833 *
Completeness [%]	83.6 *
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)
2	1	reservoir	PEG1000	5 (%(w/v))