1KAS

BETA-KETOACYL-ACP SYNTHASE II FROM ESCHERICHIA COLI

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-05
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	76.410, 76.410, 146.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.242
Rwork	0.234
R-free	0.27900
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.018
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.070	0.271
Total number of observations	141114 *
Number of reflections	18580
<I/σ(I)>	10.2	2
Completeness [%]	95.5	90.5
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	reservoir	PEG8000	27 (%)
4	1	reservoir	HEPES	0.1 (M)