1KAN

MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	78.900, 78.900, 219.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 3.000
R-factor	0.189
RMSD bond length	0.012
RMSD bond angle	0.009 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	3.000 *	3.000 *
Rmerge	0.075 *	0.209 *
Total number of observations	70244 *
Number of reflections	13914 *	3586 *
Completeness [%]	95.0 *	2243 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG8000	4 (%)
2	1	drop		100 (mM)
3	1	drop	HEPES	25 (mM)
4	1	drop		3 (mM)
5	1	drop		2.5 (mM)
6	1	reservoir	PEG8000	15.0 (%)
7	1	reservoir	HEPES	50 (mM)