1KA8

Crystal Structure of the Phage P4 Origin-Binding Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-16
Wavelength(s)	0.9794, 0.9795, 0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	82.549, 82.549, 232.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 2.950
R-factor	0.237 *
Rwork	0.241
R-free	0.28400
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	22.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	3.060
High resolution limit [Å]	2.950 *	2.950
Rmerge	0.070 *
Completeness [%]	99.0 *	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	100 (mM)	pH4.5-5.0
2	1	reservoir	ammonium sulfate	1.5-1.7 (M)
3	1	drop	protein	12 (mg/ml)