1K9W
HIV-1(MAL) RNA Dimerization Initiation Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.070, 27.320, 92.100 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.100 |
| R-factor | 0.227 * |
| Rwork | 0.227 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 14.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 3.030 |
| High resolution limit [Å] | 3.000 * | 2.900 |
| Rmerge | 0.037 * | 0.104 * |
| Number of reflections | 6955 | |
| Completeness [%] | 95.9 * | 99.4 * |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 20 * | Isopropanol, magnesium chloride, spermine, cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Isopropanol | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | cacodylate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 13 (mM) | ||
| 2 | 1 | drop | MES | 25 (mM) | pH5.6 |
| 3 | 1 | drop | sodium cacodylate | 15 (mM) | pH7.0 |
| 4 | 1 | drop | 2-propanol | 7.5 (%(w/v)) | |
| 5 | 1 | drop | spermine | 3.5 (mM) | |
| 6 | 1 | drop | RNA | 0.150 (mM) | |
| 7 | 1 | reservoir | 20 (mM) | ||
| 8 | 1 | reservoir | MES | 50 (mM) | pH5.6 |
| 9 | 1 | reservoir | 2-propanol | 15 (%(v/v)) |
