1K9D
The 1.7 A crystal structure of alpha-D-glucuronidase, a family-67 glycoside hydrolase from Bacillus stearothermophilus T-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00933 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.570, 73.570, 329.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.900 - 1.700 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | initial and partial model based on 2.4A resolution data (unpublished) |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.351 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.057 | 0.382 |
Total number of observations | 271828 * | |
Number of reflections | 94808 | |
<I/σ(I)> | 10.2 | |
Completeness [%] | 93.5 * | 60.7 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | 0.1M Na-citrate pH 5.0, 14% PEG 4000, 12% Iso-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
3 | 1 | reservoir | isopropyl alcohol | 12 (%(v/v)) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.0-5.5 |