1K92
Crystal Structure of Uncomplexed E. coli Argininosuccinate Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979055 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.699, 105.837, 127.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.600 |
| R-factor | 0.1628 * |
| Rwork | 0.163 |
| R-free | 0.17900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.522 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.042 * | 0.207 * |
| Number of reflections | 70186 | |
| Completeness [%] | 98.7 | 87.4 * |
| Redundancy | 9.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | Lemke, C., (1999) Acta Crystallogr., 55, 2028. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | pH7.5 |
| 3 | 1 | reservoir | ammonium sulfate | 1.5-1.6 (M) | |
| 4 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
