1K8I
CRYSTAL STRUCTURE OF MOUSE H2-DM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | I 4 |
| Unit cell lengths | 142.700, 142.700, 76.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.100 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25600 |
| Structure solution method | MR,MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.091 * | 0.482 * |
| Total number of observations | 63314 * | |
| Number of reflections | 13888 * | |
| <I/σ(I)> | 16.2 | 1.71 |
| Completeness [%] | 98.1 | 95.2 |
| Redundancy | 4.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 20 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 400 (mM) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 3 | 1 | drop | protein | 5-7 (mg/ml) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | MES | 50 (mM) | pH5.5 |
