1K7S
FhuD complexed with albomycin-delta 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 85.570, 85.570, 91.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.194 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.369 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.010 * | 0.646 |
| Number of reflections | 10715 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 94.8 * | 95.5 * |
| Redundancy | 94.8 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 16% PEG 4000, 0.1M Na acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.3 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 8 (%) | |
| 3 | 1 | drop | sodium acetate | 0.05 (M) | pH5.2 |
| 4 | 1 | reservoir | PEG4000 | 16 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.2 |
