1K7I
PrtC from Erwinia chrysanthemi: Y228F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.967, 101.967, 122.298 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 * - 1.590 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.20420 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.040 | 0.212 |
| Total number of observations | 577315 * | |
| Number of reflections | 98513 * | |
| <I/σ(I)> | 39.5 | 6.9 |
| Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 20 * | NaCl, pH 6.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2 (M) | ||
| 2 | 1 | reservoir | 0.1 (M) | ||
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
