1K7E
CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH N-[1H-INDOL-3-YL-ACETYL]GLYCINE ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-10 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.651, 59.081, 67.300 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.171 |
| Rwork | 0.167 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qop |
| RMSD bond length | 0.051 |
| RMSD bond angle | 3.450 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.058 * | 0.183 * |
| Total number of observations | 95120 * | |
| Number of reflections | 29178 * | |
| <I/σ(I)> | 11.3 | 2.2 |
| Completeness [%] | 91.0 | 88.7 |
| Redundancy | 3.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | PEG 8000, pH 7.80 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium bicine | 50 (mM) | pH7.8 |
| 3 | 1 | drop | sodium-EDTA | 10 (mM) | |
| 4 | 1 | drop | dithioerythritol | 1 (mM) | |
| 5 | 1 | drop | pyridoxal phosphate | 0.020 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 9-12 (%) | |
| 7 | 1 | reservoir | spermine | 1.5 (mM) | |
| 8 | 1 | reservoir | EDTA | 1 (mM) | |
| 9 | 1 | reservoir | sodium bicine | 50 (mM) | pH7.8 |
