1K7A
Ets-1(331-440)+GGAG duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-12-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.040, 75.270, 79.430 |
| Unit cell angles | 90.00, 118.28, 90.00 |
Refinement procedure
| Resolution | 17.700 * - 2.800 |
| R-factor | 0.23 * |
| Rwork | 0.224 |
| R-free | 0.27400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.040 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.251 |
| Number of reflections | 11730 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 96.9 * | 98.9 * |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 * | 293 | 1.4M-1.6M Sodium Citrate, 100mM Hepes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium citrate | ||
| 2 | 1 | 1 | Hepes |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.5 (mM) | |
| 2 | 1 | reservoir | sodium citrate | 1.4-1.6 (M) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH6.5 |
| 4 | 1 | reservoir | acetate buffer | 100 (mM) | pH4.6 |
| 5 | 1 | reservoir | PEG4000 | 20 (%) |
