1K6Z

Crystal Structure of the Yersinia Secretion Chaperone SycE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9796, 0.9795, 0.9400
Spacegroup name	P 1 21 1
Unit cell lengths	51.879, 56.038, 54.993
Unit cell angles	90.00, 114.04, 90.00

Refinement procedure

Resolution	35.000 - 2.000
R-factor	0.194 *
Rwork	0.194
R-free	0.24800
Structure solution method	MAD
Starting model (for MR)	MAD
RMSD bond length	0.006 *
RMSD bond angle	1.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.050
High resolution limit [Å]	1.950 *	2.000
Rmerge	0.050 *	0.272 *
Number of reflections	21311 *
<I/σ(I)>	16.65	3.6
Completeness [%]	99.9 *	99.7 *
Redundancy	1.90 *	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.3	300	20% PEG8000, 500 mM Imidazole Acetate pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%)
2	1	reservoir	imidazole acetate	500 (mM)	pH7.3