1K6L
Photosynethetic Reaction Center from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-19 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 141.500, 141.500, 187.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.100 |
| Rwork | 0.193 |
| R-free | 0.19400 |
| Structure solution method | Rigid-body refinement |
| Starting model (for MR) | 1pcr |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.096 | 0.281 |
| Number of reflections | 31406 * | |
| <I/σ(I)> | 10.1 | 3.5 |
| Completeness [%] | 92.0 * | 87 * |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | potassium phosphate, ldao, heptane triol, dioxane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
