1K6K
Crystal Structure of ClpA, an AAA+ Chaperone-like Regulator of ClpAP protease implication to the functional difference of two ATPase domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.021, 51.965, 65.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 1.800 |
Rwork | 0.214 |
R-free | 0.26300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ClpA N-domain |
RMSD bond length | 0.005 |
RMSD bond angle | 1.010 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.000 | |
High resolution limit [Å] | 1.800 | |
Rmerge | 0.028 * | 0.199 * |
Number of reflections | 7990 * | |
Completeness [%] | 67.2 * | 21.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 21.5-22.5 * | VAPOR DIFFUSION, HANGING DROP, pH 7.0 at 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 21.5-22.5 * | VAPOR DIFFUSION, HANGING DROP, pH 7.0 at 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 21.5-22.5 * | VAPOR DIFFUSION, HANGING DROP, pH 7.0 at 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ammonium sulfate | 60 (%) | |
2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
3 | 1 | reservoir | PIPES | 0.1 (M) | pH7.0 |
4 | 1 | reservoir | PEG8000 | 35 (%) | |
5 | 1 | reservoir | sodium citrate | 0.32 (M) |