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1K6K

Crystal Structure of ClpA, an AAA+ Chaperone-like Regulator of ClpAP protease implication to the functional difference of two ATPase domains

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X9B
Synchrotron siteNSLS
BeamlineX9B
Temperature [K]100
Detector technologyCCD
DetectorADSC QUANTUM 4
Spacegroup nameP 21 21 21
Unit cell lengths36.021, 51.965, 65.100
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution40.000

*

- 1.800
Rwork0.214
R-free0.26300

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)ClpA N-domain
RMSD bond length0.005
RMSD bond angle1.010

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]14.000
High resolution limit [Å]1.800
Rmerge0.028

*

0.199

*

Number of reflections7990

*

Completeness [%]67.2

*

21.1

*

Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5

*

21.5-22.5

*

VAPOR DIFFUSION, HANGING DROP, pH 7.0 at 294K
1VAPOR DIFFUSION, HANGING DROP7.5

*

21.5-22.5

*

VAPOR DIFFUSION, HANGING DROP, pH 7.0 at 294K
1VAPOR DIFFUSION, HANGING DROP7.5

*

21.5-22.5

*

VAPOR DIFFUSION, HANGING DROP, pH 7.0 at 294K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropammonium sulfate60 (%)
21dropHEPES20 (mM)pH7.5
31reservoirPIPES0.1 (M)pH7.0
41reservoirPEG800035 (%)
51reservoirsodium citrate0.32 (M)

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PDB entries from 2024-12-25

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