1K66

Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-20
Detector	MARRESEARCH
Wavelength(s)	0.9800, 0.9811, 0.9500, 1.05
Spacegroup name	P 41 21 2
Unit cell lengths	72.180, 72.180, 142.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.750
R-factor	0.2161
Rwork	0.216
R-free	0.24300 *
RMSD bond length	0.005
RMSD bond angle	22.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.750	1.750 *
Rmerge	0.039	0.122 *
Total number of observations	260719 *
Number of reflections	39024 *
<I/σ(I)>	27.4
Completeness [%]	98.2 *	98.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	4 *	PEG 4000, magnesium chloride, tris buffer, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 280K
1	VAPOR DIFFUSION, HANGING DROP	8 *	4 *	PEG 4000, magnesium chloride, tris buffer, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 280K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	3.75 (M)
2	1	reservoir	glycerol	5 (%(v/v))
3	1	reservoir	2-mercaptoethanol	1 (mM)
4	1	drop	protein	3-5 (mg/ml)
5	1	drop		150 (mM)
6	1	drop	Tris-HCl	10 (mM)	pH8.0
7	1	drop	glycerol	5 (%(v/v))
8	1	drop	2-mercaptoethanol	1 (mM)