1K61

MATALPHA2 HOMEODOMAIN BOUND TO DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-24
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.940, 70.240, 68.290
Unit cell angles	90.00, 105.42, 90.00

Refinement procedure

Resolution	16.010 - 2.100
R-factor	0.221
Rwork	0.221
R-free	0.27800 *
Structure solution method	MIR and molecular replacement
Starting model (for MR)	1apl
RMSD bond length	0.006
RMSD bond angle	17.300 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.048	0.287
Number of reflections	23014
<I/σ(I)>	14	2.5
Completeness [%]	98.5	81
Redundancy	2.07	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	PEG 6000, Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 6000
2	1	1	Bicine