1K61
MATALPHA2 HOMEODOMAIN BOUND TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.940, 70.240, 68.290 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 16.010 - 2.100 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27800 * |
| Structure solution method | MIR and molecular replacement |
| Starting model (for MR) | 1apl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 17.300 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.287 |
| Number of reflections | 23014 | |
| <I/σ(I)> | 14 | 2.5 |
| Completeness [%] | 98.5 | 81 |
| Redundancy | 2.07 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | PEG 6000, Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | Bicine |
