1K5J
The Crystal Structure of Nucleoplasmin-Core
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-18 |
| Detector | BRANDEIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.700, 67.100, 101.700 |
| Unit cell angles | 90.00, 123.30, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 2.300 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.25160 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.370 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.053 | 0.173 |
| Total number of observations | 250956 * | |
| Number of reflections | 30584 | |
| <I/σ(I)> | 19 | 4 |
| Completeness [%] | 96.3 | 94.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | HEPES, magnesium chloride, PEG-400, 2-propanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | PEG400 | 5 (%) | |
| 5 | 1 | reservoir | 2-propanol | 22-27 (%) | |
| 6 | 1 | reservoir | ethylene glycol | 20 (%) |
