1K5J

The Crystal Structure of Nucleoplasmin-Core

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01-18
Detector	BRANDEIS
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	116.700, 67.100, 101.700
Unit cell angles	90.00, 123.30, 90.00

Refinement procedure

Resolution	500.000 * - 2.300
R-factor	0.219 *
Rwork	0.219
R-free	0.25160
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.380 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.370
High resolution limit [Å]	2.280	2.280
Rmerge	0.053	0.173
Total number of observations	250956 *
Number of reflections	30584
<I/σ(I)>	19	4
Completeness [%]	96.3	94.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	HEPES, magnesium chloride, PEG-400, 2-propanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir		0.2 (M)
4	1	reservoir	PEG400	5 (%)
5	1	reservoir	2-propanol	22-27 (%)
6	1	reservoir	ethylene glycol	20 (%)