1K58
Crystal Structure of Human Angiogenin Variant D116H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-02-02 |
| Detector | XENTRONICS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 35.980, 35.980, 199.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.700 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.36000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ang |
| RMSD bond length | 0.012 |
| RMSD bond angle | 29.000 * |
| Data reduction software | XDS |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 * | |
| Total number of observations | 13508 * | |
| Number of reflections | 3781 * | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 92.3 | 52.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 16 * | sodium citrate, 2-propanol, PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | in water |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | 2-propanol | 20 (%(v/v)) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%(w/v)) |
