1K58

Crystal Structure of Human Angiogenin Variant D116H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1997-02-02
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	35.980, 35.980, 199.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.700
R-factor	0.191
Rwork	0.191
R-free	0.36000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ang
RMSD bond length	0.012
RMSD bond angle	29.000 *
Data reduction software	XDS
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.073 *
Total number of observations	13508 *
Number of reflections	3781 *
<I/σ(I)>	13.3
Completeness [%]	92.3	52.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6	16 *	sodium citrate, 2-propanol, PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)	in water
2	1	reservoir	sodium citrate	0.1 (M)	pH5.6
3	1	reservoir	2-propanol	20 (%(v/v))
4	1	reservoir	PEG4000	20 (%(w/v))