1K52
Monomeric Protein L B1 Domain with a K54G mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.634, 66.634, 108.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 * - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WT B1 domain pdb entry 1HZ5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.250 * |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.420 |
| Total number of observations | 1330332 * | |
| Number of reflections | 26473 | |
| <I/σ(I)> | 20.4 | 3 |
| Completeness [%] | 99.7 | 96.6 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 * | 277 | 120mM ZnOAc 50mM Cacadylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 50 (mM) | pH4.5 | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | EDTA | 2 (mM) | |
| 4 | 1 | reservoir | 120 (mM) | ||
| 5 | 1 | reservoir | cacodylate | 50 (mM) | pH6.5 |
