1K51
A G55A Mutation Induces 3D Domain Swapping in the B1 Domain of Protein L from Peptostreptococcus magnus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.008, 75.125, 60.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.800 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hz5 |
| RMSD bond length | 0.004 * |
| RMSD bond angle | 1.260 * |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.134 |
| Total number of observations | 58197 * | |
| Number of reflections | 10272 | |
| <I/σ(I)> | 31.6 | 2.9 |
| Completeness [%] | 98.6 | 95.6 |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 225mM ZnOAc, 2% PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 15 (mM) | pH7.0 |
| 2 | 1 | reservoir | 225 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 2 (%) | |
| 4 | 1 | reservoir | cacodylate | 50 (mM) | pH6.5 |
| 5 | 1 | reservoir | glycerol | 25 (%) |
