1K4V
1.53 A Crystal Structure of the Beta-Galactoside-alpha-1,3-galactosyltransferase in Complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.909 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.022, 94.145, 94.382 |
| Unit cell angles | 90.00, 98.92, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.530 |
| R-factor | 0.1405 * |
| Rwork | 0.141 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g93 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.106 * | 0.497 * |
| Total number of observations | 386939 * | |
| Number of reflections | 114924 | |
| <I/σ(I)> | 11.73 | 1.98 |
| Completeness [%] | 98.2 | 93.8 |
| Redundancy | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 16 * | PEG6000, TRIS/HCL, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | MES/NaOH | 20 (mM) | pH6.0 |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | UDP | 10 (mM) | |
| 5 | 1 | drop | 0.1 (mM) | ||
| 6 | 1 | reservoir | PEG6000 | 5 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
