1K41
Crystal structure of KSI Y57S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-18 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.889, 73.135, 51.156 |
| Unit cell angles | 90.00, 89.85, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.31900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.083 |
| RMSD bond angle | 3.172 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 * | |
| Number of reflections | 15486 | |
| Completeness [%] | 94.9 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 22 * | Sodium Acetate, Ammonium acetate, pH 4.6, EVAPORATION, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium acetate | 1.2 (M) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
