1K3S
Type III Secretion Chaperone SigE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-03 |
| Detector | BRANDEIS - B1.2 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.497, 48.725, 94.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2236 |
| Rwork | 0.224 |
| R-free | 0.27170 |
| Structure solution method | MAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.036 | 0.149 |
| Number of reflections | 15558 | |
| <I/σ(I)> | 17 | 3.1 |
| Completeness [%] | 92.4 | 63 |
| Redundancy | 11.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.8 sodium-potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
