1K3E
Type III secretion chaperone CesT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.586, 77.856, 94.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.225 * |
| Rwork | 0.225 |
| R-free | 0.28500 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.038 | 0.151 |
| Total number of observations | 43030 * | |
| Number of reflections | 10290 | |
| <I/σ(I)> | 25.6 | 6.2 |
| Completeness [%] | 94.0 | 72.2 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.2 * | 291 | 10% PEG 600, 1.5 M ammonium sulfate, 0.1 M Sodium Citrate, pH 5.6, VAPOR DIFFUSION, temperature 291.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 20 (mM) | pH8.2 |
| 3 | 1 | 1 | protein | 20 (mg/ml) | |
| 4 | 1 | 2 | ammonium sulfate | 1.3-1.8 (M) | pH5.4-6.2 |
