1K35
Crystal Structure of Phosphomannomutase/Phosphoglucomutase from P.aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979151 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.038, 73.263, 92.354 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.200 |
| R-factor | 0.23773 |
| Rwork | 0.235 |
| R-free | 0.27600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 * | 0.333 * |
| Total number of observations | 185144 * | |
| Number of reflections | 24319 | |
| <I/σ(I)> | 27.8 | 5.8 |
| Completeness [%] | 96.5 | 98.7 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Regni, C.A., (2000) Acta Crystallogr, D56, 761. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12-15 (mg/ml) | |
| 2 | 1 | drop | MOPS | 10 (mM) | pH7.0 |
| 3 | 1 | reservoir | sodium potassium tartrate | 1.4 (M) | |
| 4 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
