1K30

Crystal Structure Analysis of Squash (Cucurbita moschata) glycerol-3-phosphate (1)-acyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-09
Detector	MARRESEARCH
Wavelength(s)	1.54182
Spacegroup name	P 21 21 21
Unit cell lengths	61.134, 65.098, 103.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 - 1.900
R-factor	0.188
Rwork	0.186
R-free	0.22400
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.058	0.248
Number of reflections	32652
<I/σ(I)>	12.4	4.4
Completeness [%]	98.4	95.9
Redundancy	3.3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	290	100mM citrate buffer, 100mM ammonium acetate, 10%(v/v) 2-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	14-25 (%)
2	1	reservoir	citrate	100 (mM)
3	1	reservoir	ammonium acetate	100 (mM)
4	1	reservoir	isopropanol	10 (%(v/v))