1K30
Crystal Structure Analysis of Squash (Cucurbita moschata) glycerol-3-phosphate (1)-acyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54182 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.134, 65.098, 103.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22400 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.248 |
| Number of reflections | 32652 | |
| <I/σ(I)> | 12.4 | 4.4 |
| Completeness [%] | 98.4 | 95.9 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 100mM citrate buffer, 100mM ammonium acetate, 10%(v/v) 2-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 14-25 (%) | |
| 2 | 1 | reservoir | citrate | 100 (mM) | |
| 3 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 4 | 1 | reservoir | isopropanol | 10 (%(v/v)) |
