1K2Z
The Crystal Structure of d(GGCCAATTGG) Complexed with Distamycin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9116 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.106, 40.928, 53.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.380 |
| R-factor | 0.2135 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB entry DD0002 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.050 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.041 | |
| Number of reflections | 2351 | |
| <I/σ(I)> | 22.6 | 5.8 |
| Completeness [%] | 92.7 | 93.4 |
| Redundancy | 5.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | MPD, magnesium chloride, cacodylate, spermine, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | MgCl2 |
