1K2X
Crystal structure of putative asparaginase encoded by Escherichia coli ybiK gene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0442 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.296, 77.624, 148.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.500 - 1.650 |
| R-factor | 0.16349 |
| Rwork | 0.163 |
| R-free | 0.19836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gaw polyalanine model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.411 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.075 | 0.476 |
| Number of reflections | 70249 | |
| <I/σ(I)> | 16.8 | 2.5 |
| Completeness [%] | 89.9 | |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 292 | PEG 4000, PEG 400, magnesium chloride, Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 3 | 1 | reservoir | PEG400 | 15 (%) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) |
