1K24
Crystal Structure of the OpcA Outer Membrane Adhesin/Invasin from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.859, 46.346, 73.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.500 - 2.030 |
| R-factor | 0.235 * |
| Rwork | 0.235 |
| R-free | 0.27400 |
| Structure solution method | MIR |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.951 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 * | 2.080 |
| High resolution limit [Å] | 2.000 * | 2.030 |
| Rmerge | 0.081 * | 0.325 |
| Number of reflections | 21875 | |
| <I/σ(I)> | 16.5 | 4.1 |
| Completeness [%] | 98.3 | 82.4 |
| Redundancy | 10.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Prince, S.M., (2001) Acta Crystallogr., Sect.D, 57, 1164. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ethanol | 80 (%) | |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 25 (mM) | pH7.5 |
| 4 | 1 | drop | n-decylpentaoxyethylene | 1 (%(v/v)) | |
| 5 | 1 | reservoir | Tris acetic acid | 50 (mM) | pH7.5 |
| 6 | 1 | reservoir | zinc acetate | 150 (mM) | |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | n-heptyl beta-D-glucoside | 0.5 (%(w/v)) | |
| 9 | 1 | reservoir | PEG4000 | 20 (%(w/v)) |
