1K0M

Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-07-15
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	45.271, 55.332, 89.140
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	87.710 - 1.400
R-factor	0.138 *
Rwork	0.138
R-free	0.17799
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	1.770
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.030 *	0.080 *
Total number of observations	259290 *
Number of reflections	81729 *
<I/σ(I)>	28.9	9.3
Completeness [%]	94.1 *	94 *
Redundancy	5.2	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5	4 *	PEG MME 5K, AMMONIUM SULPHATE, SODIUM ACETATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-20 (mg/ml)
2	1	drop	n-octyl-beta-D-glucopyranoside	0.5 (%)
3	1	reservoir	PEG5000 MME	14-18 (%)
4	1	reservoir	ammonium sulfate	0-30 (mM)
5	1	reservoir	sodium acetate	0.1 (M)	pH5.0