1K0M
Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-07-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.271, 55.332, 89.140 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 87.710 - 1.400 |
R-factor | 0.138 * |
Rwork | 0.138 |
R-free | 0.17799 |
Structure solution method | MIR |
RMSD bond length | 0.018 |
RMSD bond angle | 1.770 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.030 * | 0.080 * |
Total number of observations | 259290 * | |
Number of reflections | 81729 * | |
<I/σ(I)> | 28.9 | 9.3 |
Completeness [%] | 94.1 * | 94 * |
Redundancy | 5.2 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5 | 4 * | PEG MME 5K, AMMONIUM SULPHATE, SODIUM ACETATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-20 (mg/ml) | |
2 | 1 | drop | n-octyl-beta-D-glucopyranoside | 0.5 (%) | |
3 | 1 | reservoir | PEG5000 MME | 14-18 (%) | |
4 | 1 | reservoir | ammonium sulfate | 0-30 (mM) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |