1K01
Structural Basis for the Interaction of Antibiotics with the Peptidyl Transferase Center in Eubacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-04-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 171.100, 409.300, 696.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| R-factor | 0.275 * |
| Rwork | 0.275 |
| R-free | 0.32100 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.124 * | 0.577 * |
| Number of reflections | 238661 | |
| Completeness [%] | 88.5 | 71 |
| Redundancy | 3.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 291 | ethanol, dimethylhexanediol, MgCl2, KCl, Hepes, NH4Cl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ethanol | ||
| 2 | 1 | 1 | dimethylhexanediol | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 1 | Hepes | ||
| 6 | 1 | 1 | NH4Cl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 10 (mM) | ||
| 2 | 1 | reservoir | 60 (mM) | ||
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | HEPES | 10 (mM) |
