1JZQ

Isoleucyl-tRNA synthetase Complexed with Isoleucyl-adenylate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	FUJI
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	160.470, 95.190, 126.210
Unit cell angles	90.00, 126.22, 90.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.22 *
Rwork	0.220
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.470 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.000
Rmerge	0.096 *	0.357 *
Total number of observations	63934 *
Number of reflections	27250
Completeness [%]	88.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	Nureki, O., (1998) Science, 280, 578. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	12 (%)
3	1	reservoir	isopropanol	6 (%)
4	1	reservoir	MPD	1 (%)
5	1	reservoir	HEPES	60 (mM)