1JZF

Pseudomonas aeruginosa Oxidized Azurin(Cu2+) Ru(tpy)(phen)(His83)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.03
Spacegroup name	I 2 2 2
Unit cell lengths	52.776, 61.441, 75.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.500
R-factor	0.217 *
Rwork	0.218
R-free	0.22100 *
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.038 *
Number of reflections	19968
<I/σ(I)>	32	0.118
Completeness [%]	98.9 *	98.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	293	PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	imidazole	40 (mM)
3	1	drop		2 (mM)	pH7.2
4	1	reservoir	imidazole	100 (mM)	pH6.0-8.0
5	1	reservoir		100 (mM)
6	1	reservoir		6.25 (mM)
7	1	reservoir	PEG4000-8000	25-38 (%)