1JZE
Pseudomonas aeruginosa Azurin Ru(bpy)2(im)(His83)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.430, 61.760, 68.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.600 |
R-factor | 0.249 * |
Rwork | 0.249 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bex |
RMSD bond length | 0.030 * |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.050 * | |
Number of reflections | 13982 | |
<I/σ(I)> | 34 | 2.9 |
Completeness [%] | 92.1 | 46.1 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 293 | PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | drop | imidazole | 40 (mM) | |
3 | 1 | drop | 2 (mM) | pH7.2 | |
4 | 1 | reservoir | imidazole | 100 (mM) | pH6.0-8.0 |
5 | 1 | reservoir | 100 (mM) | ||
6 | 1 | reservoir | 6.25 (mM) | ||
7 | 1 | reservoir | PEG4000-8000 | 25-38 (%) |