1JZD

DsbC-DsbDalpha complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	68.910, 68.910, 230.336
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 2.300
Rwork	0.234
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eej (DsbC monomer)
RMSD bond length	0.012
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	1.900	2.300
Rmerge	0.067
Number of reflections	24849
<I/σ(I)>	7.4
Completeness [%]	97.6	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.9	291	PEG, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.9	291	PEG, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.9	291	PEG, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K