1JZ4
E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-DEOXY-GALACTOSYL-ENZYME INTERMEDIATE (Low Bis-Tris)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-01 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 149.500, 169.000, 200.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.100 |
R-factor | 0.164 * |
Rwork | 0.164 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dp0 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.081 | 0.270 |
Number of reflections | 264902 | |
<I/σ(I)> | 7.5 | 2.3 |
Completeness [%] | 90.0 | 80.2 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 288 | used macroseeding, Juers, D.H., (2000) Protein Sci., 9, 1685. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
10 | 1 | reservoir | dithiothreitol | 10 (mM) | |
2 | 1 | drop | Bis-Tris | 100 (mM) | pH6.5 |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | 5 (mM) | ||
6 | 1 | reservoir | PEG8000 | 10 (%) | |
7 | 1 | reservoir | bis-Tris | 100 (mM) | pH6.5 |
8 | 1 | reservoir | 200 (mM) | ||
9 | 1 | reservoir | 100 (mM) |